Temporary

Part:BBa_J70575:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-05-09)


{15,18;13,10} BsaXI BioScaffold v 5.0, no BsaXI, V169I in E0040


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 751
    Illegal AgeI site found at 863
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

this removes BsaXI site from R0010 and incorporates a PCR mutation that occurred when trying to amplify J70568


Source

designed (pcr'd from J04450)

J70568f: gtttcttcgaattcgcggccgcttctagagctccgattcattaatgcagctggcacgac

J70568r: gtttcttcctgcagcggccgctactagtacctgtcctcctataaacgcagaaag

References